qPCR raw fluorescence datasets (Excel files) for testing methods and algorithms
- 'reps': S27a, cDNA from testicular biopsies, seven 10-fold dilutions, 4 replicates, Lightcycler 1.0 with SybrGreen chemistry. Download. RefLink.
- 'reps2': S27a, 2 cDNAs from testicular biopsies, five 4-fold dilutions, 3 replicates, Lightcycler 1.0 with SybrGreen chemistry. Download. RefLink.
- 'reps3': S27a, cDNAs from mesenchymal stem cells, six 4-fold dilutions, 3 replicates, MX3000P with SybrGreen chemistry. Download. RefLink.
- 'guescini1': NADH dehydrogenase 1, from cloned plasmid, seven 10-fold dilutions, 12 replicates, Lightcycler 480 with SybrGreen chemistry. Download. RefLink.
- 'guescini2': NADH dehydrogenase 1, from cloned plasmid, five different mastermix dilutions, 12 replicates, Lightcycler 480 with SybrGreen chemistry. Download. RefLink.
- 'rutledge': K2/K1 primer, on purified amplicon, six 10-fold dilutions, 20 replicates, Opticon2 with SybrGreen chemistry. Download. RefLink.
- 'S27': S27a, cDNA from 15 different testicular biopsies, single runs, no replicates, Lightcycler 1.0 with SybrGreen chemistry. Download. RefLink.
- 'batsch1': rat SLC6A14, cDNA from frozen tissue, five 4-fold dilutions, 3 replicates, Lightcycler 1.0 with TaqMan chemistry. Download. RefLink.
- 'batsch2': human SLC22A13, cDNA from frozen tissue, five 4-fold dilutions, 3 replicates, Lightcycler 1.0 with TaqMan chemistry. Download. RefLink.
- 'batsch3': pig EMT, cDNA from frozen tissue, five 4-fold dilutions, 3 replicates, Lightcycler 1.0 with TaqMan chemistry. Download. RefLink.
- 'batsch4': chicken ETT, cDNA from frozen tissue, five 4-fold dilutions, 3 replicates, Lightcycler 1.0 with SybrGreen chemistry. Download. RefLink.
- 'batsch5': human GAPDH, cDNA from frozen tissue, five 4-fold dilutions, 3 replicates, Lightcycler 1.0 with SybrGreen chemistry. Download. RefLink.
- 'sisti1': human MT-ND1, from cloned plasmid, six 10-fold dilutions, 12 replicates, Lightcycler 480 with SybrGreen chemistry. Download. RefLink.
- 'sisti2': human MT-ND1, from cloned plasmid, 9 runs with 2-fold dilutions of tannic acid as inhibitor, 6 replicates, Lightcycler 1.0 with SybrGreen chemistry. Download. RefLink.