Fitting a 5-parameter
sigmoidal model
Fitting replicates into a
'replist' and plotting all
Fitting replicates into a
'replist' and display error bars
Analysis of qPCR efficiency
and threshold cycles
Outlier detection and fitting
an exponential model
Model selection by nested
f-tests or Akaike weights
Batch calculation of
many qPCR runs
Calibration curves
and new samples prediction
Examining the fit in
the exponential region
Error propagation with
Monte Carlo simulation
Permutation of class labels
similar to REST software
Averaging a batch of
reference PCRs
'Window-of-linearity' method with baseline optimization
Bootstrapping/jackknifing
qPCR data => confidence
Simulating qPCR data
PRESS statistic
Cy0 value as in Guescini et al.
Finding non-sigmoidal structures (failed runs)
Melting curve analysis and automatic Tm detection
Batch ratio analysis using three methods (MC, Perm, Prop)
7-parameter model and residuals plot
'mak3' mechanistic model from Boggy et al. (2010)
'Linear regression of efficiency' with baseline optimization
"heatmap"-like image plot for high-throughput data