
Fitting a 5parameter
sigmoidal model 
Fitting replicates into a
'replist' and plotting all 
Fitting replicates into a
'replist' and display error bars 
Analysis of qPCR efficiency
and threshold cycles 
Outlier detection and fitting
an exponential model 
Model selection by nested
ftests or Akaike weights 
Batch calculation of
many qPCR runs 
Calibration curves
and new samples prediction 
Examining the fit in
the exponential region 
Error propagation with
Monte Carlo simulation 
Permutation of class labels
similar to REST software 
Averaging a batch of
reference PCRs

'Windowoflinearity' method with baseline optimization 
Bootstrapping/jackknifing
qPCR data => confidence 
Simulating qPCR data

PRESS statistic

Cy0 value as in Guescini et al.

Finding nonsigmoidal structures (failed runs)

Melting curve analysis and automatic Tm detection

Batch ratio analysis using three methods (MC, Perm, Prop)

7parameter model and residuals plot

'mak3' mechanistic model from Boggy et al. (2010)

'Linear regression of efficiency' with baseline optimization

"heatmap"like image plot for highthroughput data

'cm3' mechanistic model from Carr & Moore (2012)

3DPlot of a qPCR batch
